The expression and purification of rGFP started with the growth of the bacterial culture and ended with the elution of rGFP off the Ni 2-agarose column. Assuming the column was not overloaded, which of the following changes to the procedure would result in an increase in the yield of rGFP in the elution fractions? A. Use a 1ml bed volume instead of a 0.5ml bed volume.
B. Induce the culture longer than 3 hours before harvesting.
C. Increase the number of washes before switching to elution buffer.
D. Use a 20ml pellet instead of a 15 ml pellet.

the correct answer is b. possesses the characteristic of compartmentalization of organelles, wherein the organelles are bound by membranes.

explanation:

it is either c or d and i know for sure it is not a or b

the two answers are a, the population of birds with large beaks will increase on the island, and c, the trait for large beaks will be passed on to future generations through natural selection. the reason for that is because if the birds that have beaks developed for eating small seeds lose all of their food, they will go hungry and eventually starve because they physically cannot eat the large seeds, therefore making the population of large beaked birds higher. also, if the trait of large beaks are dominant on the island over small beaks, then over time the large beaked birds will reproduce more and more, while the smaller beaked birds will reproduce less and less because they can't survive with the food provided. that leads to the trait of small beaks disappearing and the trait of large beaks taking over the island.