Over the past 60 years, many amphibian species have experienced significant population declines and some species have become extinct. Scientists suspected that local human activities such as the destruction of wetlands, regional pollution, and deforestation were the main reasons for these losses. However, research over the past 20 years reveals significant amphibian population declines in protected areas of the world, such as nature preserves and parks. These global declines suggest widespread problems including increased ultraviolet radiation, acid rain, and disease. In Switzerland, for example, 14 of the 20 native amphibian species are threatened with extinction. Some biologists urge the collection of the few remaining individuals of some of the most threatened amphibian species, to preserve them if they become extinct in the wild. If such captive breeding programs could produce thousands of individuals from just a few of the remaining survivors, the species will still be threatened because of .