When performing the streak for isolation technique, your result is unexpected: There is only growth obtained in quadrant 1. The growth observed in quadrant 1 is as expected, heavy with no isolated colonies. However, no growth is visible in any other quadrants. Which of the following errors could explain this unexpected result?
A) The loop did not cross into quadrant 1 when streaking quadrant 2
B) You forgot to flame the loop in between quadrants
C) All of the errors listed here could be responsible for this unexpected result
D) Not enough bacteria was obtained from the starting culture when streaking quadrant 1

A) The loop did not cross into quadrant 1 when streaking quadrant 2

Explanation:

The streak for isolation technique is a method used to get isolated bacterial colonies. This technique consists of, in a petri dish with culture medium, where, with the help of an inoculation loop, the bacteria are scratched on one side of the dish. This side is called the first quadrant. This inoculation loop must then cross with the first quadrant and be dragged to the second quadrant, where new lines must be made. This allows a part of the bacteria from the first quadrant to be carried over to the second. The inoculation loop must cross the second quadrant again to inoculate the third quadrant, and so on.

In this case, we can see that for bacterial colonies to grow in all quadrants, it is essential that the loop crosses in the first quadrant to cross out the second quadrant. If not, only the first quadrant will have bacterial colonies.

A) The loop did not cross into quadrant 1 when streaking quadrant 2

Explanation:

There are many different techniques to isolate bacteria populations. Using solid media such as agar, you can perform the streak for isolation technique.

What you want to do by using this technique is to get an isolated colony of the bacteria of interest. And to get it, you need to dilute a sample or inoculum of bacteria as much as possible by streaking it. Every time you do it, you take fewer amounts of cells from the original sample, separating them each time more, to the point of having only one single cell apart by a few millimeters from another cell. The isolated cell divides and forms a new pure colony.

So basically, you need to imaginary divide the agar plate into four quadrants.

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