In reverse genetics, what is the correct order in which the experimenter proceeds? A. identification of a gene of interest followed by screening for mutations in that gene and studies of the phenotypes caused those mutations. B. selection of mutants with a phenotype of interest and then mapping to locate the effected gene(s) C. silencing the genes in question using RNAi, followed by mapping to locate the gene D. random mutagenesis, followed by observation of offspring for newly acquired traits

The empty trna moves off and picks up another matching amino acid from the cytoplasm in the cell. the anticodon of the trna, with its attached amino acid, pairs to the codon of the mrna, which is attached to a ribosome. this sequence is repeated until the ribosome reaches a stop codon on the mrna, which signals the end of protein synthesis. the ribosome forms a peptide bond between the amino acids, and an amino acid chain begins to form. when a second trna with its specific amino acid pairs to the next codon in sequence, the attached amino acid breaks from the first trna and is bonded to the amino acid of the second trna.