You have modified the green fluorescent protein (GFP) to carry a nuclear localization signal at its N-terminus and a nuclear export signal at its C-terminus. When this protein is expressed in cells, you find that about 60% of it is located in the nucleus and the rest in the cytoplasm and that its rates of import and export determine the observed distribution. Nevertheless, you are concerned that the modified GFP might not fold properly even though it retains its fluorescent properties. Specifically, you worry that the 40% in the cytoplasm is not being transported at all because neither signal is exposed, and the 60% in the nucleus has only the nuclear import signal available. To test this, you may treat cells with leptomycin B, a drug that inhibits nuclear export. How might addition of leptomycin B to such cells resolve this issue

Complete the sentences by matching the names of trees to the appropriate blanks. to do this, drag the names on the left into the appropriate blanks in the sentences on the right. match the words in the left column to the appropriate blanks in the sentences on the right. terms may be used more than once. make certain you have filled every blank with a term before clicking submit. 1. deciduous trees lose their leaves seasonally and include trees such as maple and elm. 2. conifer leaves are generally thin and needle-like, while seeds are contained in cones. conifers include cedar and pine. 3. evergreens are plants that maintain their leaves in all seasons and include trees such as cedar, elm, and pine. 4. hardwoods reproduce using flowers and have broad leaves; hardwoods include trees such as maple, , and cedar. elm returned you filled in 2 of 10 blanks incorrectly. mango trees are tropical broad leaf plants that maintain their leaves all year long. no credit lost. try again.

How is transcription similar to translation in terms of base pairing?

What function does the limb sever in each animal? how are the limb bones of the four animals similar? based on these similarities what can be inferred about the evolution of the four animals.