Enzymes play essential roles in many functions of cells, organs and organisms. The diagram below represents a hypothetical enzyme protein with 100 amino acids. The number scale indicates the position of each amino acid. Below the protein representation is an annotation that describes the function of the indicated amino acids in this protein. This enzyme is regulated by an allosteric inhibitor. Amino Acid position number>

0 10 20 30 40 50 60 70 80 90 100



I<>I I<>I
Allosteric Site Active Site

The questions are below.

Please organize your answers using the headings associated with each question below. To do so, simply copy the four questions and their subheadings into the answer space below and then type your answers to the parts of each question in alignment with its corresponding heading.

A. Identify an amino acid position for this enzyme where, if there were a point insertion mutation in the codon, the most likely effect on the enzyme would be neutral. Justify your answer with a brief description.

Justification:

B. What would be the likely effect on this enzyme’s activity if there were two separate deletion mutations and two insertion mutations and one substitution mutation in the codons for the amino acids between position 45 and 65? Justify your answer with a brief description.

Justification:

C. Where would a substitution mutation in the second base of a codon be least likely to have an effect on the function of the enzyme? Justify your answer with a brief description.

Justification:

D. What would be the likely effect on enzyme function if there were a substitution mutation in the first base of a codon for an amino acid at position 30? Justify your answer with a brief description.

Justification: