A new variant of the laboratory strain E. coli has been isolated and requires oxygen for growth. You want to determine what metabolic pathways are active in this organism. It does not accumulate any carbon polymer. Based on what you know about metabolites present in and compounds synthesized by bacteria (such as cell walls and phospholipids), for each of the three carbon sources, WRITE THE LETTER OF THE METABOLIC PATHWAY and/or REACTION (located bottom ) that is required for the organism to metabolize the carbon source and use it as biosynthetic precursor for long-term and sustained growth of the organism, requiring oxygen.

CARBON SOURCES:

I. Fructose

II. C20 saturated Fatty Acid

III. Gluconate (gluconic acid)

METABOLIC PATHWAY and/or REACTION:

A. Embden-Meyerof-Parnas (EMP) Aerobic glycolysis

B. Entner-Doudoroff Pathway (ED)

C. PDH (pyruvate dehydrogenase)

D. Tricarboxylic Acid Cycle (TCA cycle)

E. Electron Transport Chain (ETC) and ATP synthase

F. Glyoxylate Cycle

G. Gluconeogenesis (GNG)

H. Beta-oxidation

I. Fatty Acid Synthesis

J. Lactic Acid Fermentation

K. Pyruvate Carboxylase

L. Pentose Phosphate Pathway