According to darwin, organisms with traits that are best suited for the environment will

a) not pass those traits on to future generations
b) die before they are able to mate
c) pass those traits on to future generations
d) not be able to have offspring

answer: - few nutrients

high pressure

low temperatures

explanation: 1. few nutrients:   open-ocean zone is located way far from the land, which is the main source of the essential nutrients.2. high pressure: pressure increases by 1 atmosphere for every 10 meters increase in depth.3. ample sunlight: a large fraction of the sunlight is reflected back to the atmosphere from the sea surface. 4. varying salinity: below the thermocline, the water is isolated from the atmosphere so the salinity remains stable over the year.5. low temperatures:   the temperature of open-ocean zone ranges from a low of -2°c to an average of 17°c

this protocol describes a detailed procedure for intracellular flow cytometry staining.