How do humans obtain the nitrogen they use in their bodies?
1. by consuming plants or other animals
2. by absorbing ammonia from bacteria growing in the roots of plants
3. by breathing the nitrogen present in the atmosphere
4. by absorbing nitrogen found in the soil

The dna in a cell’s nucleus encodes proteins that are eventually targeted to every membrane and compartment in the cell, as well as proteins that are targeted for secretion from the cell. for example, consider these two proteins: phosphofructokinase (pfk) is an enzyme that functions in the cytoplasm during glycolysis. insulin, a protein that regulates blood sugar levels, is secreted from specialized pancreatic cells. assume that you can track the cellular locations of these two proteins from the time that translation is complete until the proteins reach their final destinations. for each protein, identify its targeting pathway: the sequence of cellular locations in which the protein is found from when translation is complete until it reaches its final (functional) destination. (note that if an organelle is listed in a pathway, the location implied is inside the organelle, not in the membrane that surrounds the organelle.)

Examine the two squirrel populations in the accompanying figure. the populations are separated by a geographic barrier. if after a long period of time the two species are no longer separated, what evidence is needed to determine if speciation has occurred? the figure shows two populations of squirrels separated by a geographic barrier. examine the two squirrel populations in the accompanying figure. the populations are separated by a geographic barrier. if after a long period of time the two species are no longer separated, what evidence is needed to determine if speciation has occurred? the figure shows two populations of squirrels separated by a geographic barrier. polyploidy is creating new species. the two populations are not interbreeding freely. hybrid offspring of the two populations begin to appear. one species will increase into a population size twice as large as the other species.

To answer this question, researchers studied populations of the dusky salamander (desmognathus ochrophaeus) living on different mountain ranges in the southern appalachian mountains. the researchers tested the reproductive isolation of pairs of salamander populations by leaving one male and one female together and later checking the females for the presence of sperm. four mating combinations were tested for each pair of populations (a and b)—two within the same population (female a with male a and female b with male b) and two between populations (female a with male b and female b with male a). the proportion of successful matings for each mating combination was measured. for example, when all the matings of a particular combination were successful, the researchers gave it a value of 1; when none of the matings were successful, they gave it a value of 0. then the researchers calculated an index of reproductive isolation that ranged from 0 (no isolation) to 2 (full isolation). the reproductive isolation value for two populations is the sum of the proportion of successful matings of each type within populations (aa + bb) minus the sum of the proportion of successful matings of each type between populations (ab + ba). the table provides data for the geographic distances and reproductive isolation values for 27 pairs of dusky salamander populations.