Explain why haeiii is unlikely to be used to create recombinant dna. how does this enzyme differ from the others described in the table?

Further explanation

Recombinant DNA or rDNA is a form of artificial DNA that is made by combining or combining two or more strands of DNA strands that are normally unpaired or occur together. On the subject of molecular biology, genetic modification is carried out by inserting relevant DNA into the DNA of living organisms, for example in bacterial plasmids, to encode a particular characteristic such as antibiotics and other traits. This is different from the concept of recombinant DNA in which the combination of DNA does not occur naturally in cells but is engineered. DNA recombination process that is commonly done is by combining DNA strands from two different organisms. The joining of two DNAs from different organisms, for example in a bacterial plasmid, is assisted by the enzyme ligase. Recombinant DNA technology through DNA cutting techniques is one time reinforcing evidence that shows that DNA is a unit of inheritance.

The discovery of recombinant DNA technology has made genetic engineering analysis and development increasingly developed, especially in fields that have long practiced genetic modeling and analysis such as medicine, agriculture, and industry. Gene transfer between organisms today can be carried out even between organisms that were biologically formerly unable to join. The gene transfer process that conventionally uses sexual reproduction ie crossing can be avoided, for example in the production of "Bt" maize, one of which is composed of bacterial genes that are transferred to corn plants to make corn resistant to borer pests in Europe. Molecular markers are available to make the selection process more effective and efficient.

In the world of health, the first commercial product produced with recombinant DNA technology is insulin. This insulin research and project began before government regulations and policies regarding the widespread and commercial use of recombinant DNA were made. As basic research on recombinant DNA technology continues to seek efficiency and benefits for humanity, the commercial use of products produced from this technology is controlled by market behavior and investment capital provided.

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Recombinant DNA :

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Class: high school

Subject: biology

Keywords : Recombinant, genetics, product, technology, organism

HaeIII is not used to create a recombinant DNA; this is because it is a four-base cutter that produces a short fragment.

Further Explanation:

Restriction enzymes recognize and cleave specific DNA sequences generating either blunt or sticky ends. Restriction enzyme sequences are generally four to eight nucleotides in length and identify one restriction site. Sticky ends are made when the restriction enzyme cleaves on one strand of DNA, leaving overhangs that can base pair with the vector genome. Blunt ends are generated when restriction enzyme cleaves on both strands of DNA, leaving no overhangs. These require the enzyme ligase to join the DNA sequence to the vector sequence.

HaeIII is an endonuclease restriction enzyme and isolated from bacteria named Haemophilus aegyptius bacteria. HaeIII is four base pair cutters that used to make small fragments of the plasmid. Small pieces can easily coil in itself and not very efficient in making linear fragments.

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Answer Details:

Grade: High School

Subject: Health

Chapter: Molecular Biology

Keywords:

Restriction enzyme, DNA, restriction enzyme, blunt, sticky end, length, restriction site, linear fragments, endonuclease, efficient, small fragment.

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