PLS HELP WILLING TO GIVE BRAINLIEST

1.  In primary succession, newly exposed or newly formed rock is colonized by living things for the first time. In secondary succession, an area previously occupied by living things is disturbed then recolonized following the disturbance.

2.  Primary and secondary succession occur after both human and natural events that cause drastic change in the makeup of an area.

3.  Volcanic eruptions, Retreat of glaciers, Flooding accompanied by severe soil erosion, Landslides, Nuclear explosions, Oil spills.

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.

the actual answer was 160934