Biology, 02.04.2021 04:20 maddiefoley17

A student wants to use western blotting to confirm the presence of human Sonic hedgehog (Shh) in samples run on SDS-PAGE and transfered to a nitrocellulose membrane. The antibody reagent box in their lab contains Mouse anti-Shh MAb and Chicken anti-mouse IgG-HRP. What substrate will they need in order to perform colorimetric detection of Shh on their western blot

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A student wants to use western blotting to confirm the presence of human Sonic hedgehog (Shh) in sam...