Which step in the Gram stain is the critical step in differentiating Gram negative cells from Gram positive cells?

The rapid decolorization with ethanol or acetone

and counterstaining with safranin

When a decolorizer such as alcohol or acetone is added, it interacts with the lipids of the cell membrane. A gram-negative cell loses its outer lipopolysaccharide membrane, and the inner peptidoglycan layer is left exposed: when safranin is added they will turn red. Conversely, gram-positives retain the Cristal violet and will color in purple

step 1 ammonium can be absorbed by plants, but some is converted into nitrates which are better for plants to absorb. this is called nitrification. 2. step 2 the deer eats the plants and uses the nitrogen-containing proteins for cell growth. 3. step 3 bacteria in the soil conduct nitrogen fixation to convert nitrogen gas from the atmosphere into ammonium. 4. step 4 some of the nitrates are absorbed back into plants, but denitrification breaks down the nitrates into nitrogen gas released back into the atmosphere. 5. step 5 nitrogen is found in the urine, which gets broken down into ammonium through the process of ammonification. 6. step 6 plants absorb the ammonium and begin assimilation, the process to add the nitrogen to protein.

idk im sorry.