A fellow student attempts to follow the protocol. They know that the GCE that was applied to the column was fluorescing, however fractions E1-E10 do not fluoresce. What two student errors could have occurred during the Ni 2 Agarose purification procedure that resulted in this result?

Astudent sees several ants walking up a wall following the exact same trail that an ant took earlier. she wants to apply the scientific method to determine how the ants detected the trail. which of these steps would come first in her application of the scientific method? perform an experiment by cleaning the scent away from part of the trail. draw a conclusion that the ants follow a scent trail. make a prediction about what the ants will do after she cleans away part of the trail. hypothesize that the ants are following a scent trail that the first ant left.