Put the following 6 steps in order to indicate the overall experimental flow. a. Quantify extracted protein
b. Transform BL21(DE3) w/ pDHFR
c. Run purified protein on gel to verify by SDS-PAGE and Western Blot
d. Generate pDHFR expression vector
e. Induce DHFR protein synthesis in E. coli with IPTG
f. Purify DHRF protein by affinity chromatography (IMAC)

Ascientist wants to determine the effect of a new type of gasoline. he fillsone car with normal gasoline and another identical car with the new gasoline. which is the control group? a. the new type of gasolineb. the car with the normal gasolinec. the car with the new gasolinethe amount of gasoline usedd. the amount of gasoline used

Consider the motion of the balloon and its air contents in terms of momentum. in step 1 above, the total momentum of the balloon and its contents was zero. recall that momentum = mv. both the balloon and the air inside it had a velocity of zero and therefore the total momentum was zero. now think about what happened when the air escaped from the balloon. a certain mass of air accelerated in one direction. in order to keep the total momentum of the system zero, the balloon itself (which has mass) had to accelerate in the opposite direction. use this scenario to you explain why the soda can rotates when the water squirts out of the escape holes. what was your hypothesis concerning the water-filled can? according to your data, do you think your hypothesis was correct? (be sure to refer to your data when answering this question.) summarize any difficulties or problems you had in performing the experiment that might have affected the results. describe how you might change the procedure to avoid these problems. give at least one more example of newton's third law in everyday life.

Easy 6th grade work! 100 points! i need fast! compare the parts of a cell and the cell as a whole to another common nonliving system (i. e., a car, a city, describe the parts of a cell and their primary function.