Gel Electrophoresis 1. This technique is used to sort pieces of DNA by:

2. What other type of molecules can be sorted using this method?

3. What is used to move the DNA pieces through the gel?

4. Which pieces move the fastest & furthest?

5. What supplies do you need to make the gel?

6. What is agarose made from?

7. What is a buffer and why is it added?

8. How long does the gel take to solidify, and what happens as it cools?

9. What are the 2 reasons that you add buffer to the DNA sample to be tested?

10. Why do you use a DNA size standard?

11. What is your proof that the current is flowing through your buffer

12. What do we use to stain DNA?

Keeping in mind the life cycle of bacteriophages, consider the following problem: during the reproductive cycle of a temperate bacteriophage, the viral dna inserts into the bacterial chromosome where the resultant prophage behaves much like a trojan horse. it can remain quiescent, or it can become lytic and initiate a burst of progeny viruses. several operons maintain the prophage state by interacting with a repressor that keeps the lytic cycle in check. insults (ultraviolet light, for example) to the bacterial cell lead to a partial breakdown of the repressor, which in turn causes the production of enzymes involved in the lytic cycle. as stated in this simple form, would you consider this system of regulation to be operating under positive or negative control?

What processes would you expect to be key in the production of yogurt ?

Abiologist counts the number of rabbits in a population each year and observes a decrease in population. since the coyote population has exploded, the biologist concludes that the coyote population has had a negative interaction with the rabbit population. which describes the biologist’s actions? a.) experimentationb.) inferencec.) observationd.) interacting