An investigation was performed to determine the effect of temperature on the activity of the enzyme catalase, which breaks down hydrogen peroxide releasing oxygen. Six test tubes containing enzyme and substrate were maintained at different temperatures. Observations made during the investigation were used to construct the data table below. An increase in the rate of bubbling in each of tubes 1 through 5 would most likely be noticed after * 1)increasing the temperature of each tube to 80°C
2)increasing the enzyme concentration in each tube
3)increasing the pH in each tube
4)decreasing the temperature of each tube to –10°C