The generation of a knock-out organism generally requires all the following EXCEPT . The generation of a knock-out organism generally requires all the following EXCEPT . knowledge of the sequence product embryonic stem cells knowledge of the target sequence a targeting vector

knowledge of the sequence product

Explanation:

A gene knockout is a technique used in molecular genetics to deactivate target genes in an organism in order to study their functions by reverse genetics (i.e., gene loss). Knockouts are generated by different methods including, for example, homologous recombination or site-specific nucleases (zinc-fingers, TALENS, CRISPR/Cas9). These techniques require to know a priori the sequence of each gene to be knocked out in order to target desired mutations. In the last years, the CRISPR/Cas9 tool has gained attention to knockout genes of interest because it is a genome editing system that can be easily used for deletion or insertion of bases.

the relative standard reduction potential of the half-cell in which reduction occurs; more positive than the other half-cell. reduction is a chemical process in which electrons are added to an atom or an ion; it always occurs accompanied by oxidation of the reducing agent.

acceleration = 18/3 = 6 m/s per second.

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