31. The graph below shows the effect of tempera- ture on the relative rate of action of enzyme X
on a protein
Relative rate
of action
10 20 30 40 50 60 70 80
Temperature (*)
Which change would not affect the relative
rate of action of enzyme X?
(1) the addition of cold water when the
reaction is at 50°C
(2) an increase in temperature from 70°C to 80°C
(3) the removal of the protein when the
reaction is at 30°C
(4) a decrease in temperature from 40°C to 10°C