PCR amplification utilizes template to produce a product. For this question, select the answer that best describes the process as a whole: the nature of the template you used and the nature of the final product produced in your PCR analysis that you ran out on a gel will apply here. (Ref: slide 5

Single stranded template to produce a double stranded product.

Explanation:

Polymerase chain reaction (PCR) is a technique in the laboratory that is used to make many copies (millions or billions) of a particular region of deoxyribonucleic acid (DNA).

The reaction utilizes primers which are short pieces of single stranded oligonucleotide templates to yield a double stranded DNA.

Two primers are used in each PCR reaction, and they are designed to identify the target region to amplified they bind to opposite strands of the template DNA at the edges of the region to be copied. The template DNA is copied and a complementary DNA is produced. The end product of the reactions a double stranded DNA that is produced from the template dna. The new DNA is the exact copy of the template dna.

1. a tropical dry forest carries out more rapid nutrient cycling

step 1: initiation

initiation is the beginning of transcription. it occurs when the enzyme rna polymerase binds to a region of a gene called the promoter. this signals the dna to unwind so the enzyme can ‘‘read’’ the bases in one of the dna strands. the enzyme is now ready to make a strand of mrna with a complementary sequence of bases.

step 2: elongation

elongation is the addition of nucleotides to the mrna strand. rna polymerase reads the unwound dna strand and builds the mrna molecule, using complementary base pairs. there is a brief time during this process when the newly formed rna is bound to the unwound dna. during this process, an adenine (a) in the dna binds to an uracil (u) in the rna.

step 3: termination

termination is the ending of transcription, and occurs when rna polymerase crosses a stop (termination) sequence in the gene. the mrna strand is complete, and it detaches from dna.