Calculate the volume of plasmid and insert needed for a ligation reaction using the specifications for NEBL T4 DNA ligase (0.020 pmol vector, 0.060 pmol insert). This reaction is ligating a 3kb vector (25ng/μL) and a 2kb (10ng/μL) insert together, and the fragments have complementary sticky ends. Round final answers to the nearest hundredths place.

Indica el nombre y la fucion de los vasos sanguineos que entran y salen de los riñones

What drives an advertising campaign?

Producing disposable grocery bags consumes resources. recycling disposable bags is a solution that can recover some of the resources used to make new bags. which of the following behaviors could further refine the solution? a. disposing of bags by burning them b. reusing bags whenever possible c. using bags at once so that they do not break d. using only nonrenewable materials to make new bags subject is environmental science for apex users