Help please! DNA Assignment Instructions: For this assignment, you will replicate DNA to make a complimentary strand of DNA. Then transcribe the complimentary DNA sequence into mRNA. Next, translate the mRNA molecule into the codes for specific amino acids using the amino acid chart on the last page. Lastly, address the reflection questions.

DNA Replication:
Replicate the following strand of DNA:
Original
DNA
A
T
G
A
A
C
C
A
T
T
C
A
G
T
A
T
G
G
Complimentary DNA

Transcription:
Transcribe the DNA to make an mRNA molecule
Complimentary DNA

mRNA Molecule

Translation:
Translate the mRNA into the corresponding amino acids.
mRNA Molecule

Amino Acid

Using the DNA sequence provided, determine the amino acids.
Original
DNA
A
T
G
G
G
T
C
T
A
G
C
G
A
A
A
G
A
T
Complimentary DNA

mRNA Molecule

Amino Acid

Questions:
Using what you have learned in the lesson and the experiment, answer the following questions in complete sentences.
What is the function of mRNA?
What is the function on tRNA?
Describe what happens during transcription.
Describe what happens during translation.
Explain how DNA is used to make a protein.

DNA Replication:

Replicate the following strand of DNA:

Original DNA: A T G A A C CA T T C A G T A T G G

Complementary DNA:

Remember that the complimentary base pairs in DNA are Adenine-Thymine and Guanine-Cytosine, or A-T and G-C. This means that if the original sequence is:

A T G A A C C A T T C A G T A T G G

Then the compliment is (replace with the opposite base pairs):

T A C T T G G T A A G T C A T A C C

Transcription:

Transcribe the DNA to make an mRNA molecule

COMPLIMENTARY DNA:

mRNA Molecule:

So if our complimentary DNA (antisense stand) is T A C T T G G T A A G T C A T A C C then to transcribe, we basically create another complimentary strand, but for mRNA, we use Uracil instead of Thymine:

A U G A A C C A U U C A G U A U G G  

Translation:

Translate the mRNA into the corresponding amino acids.

mRNA MOLECULE:

AMINO ACID:

Using the DNA sequence provided, determine the amino acids.

Next, take each codon (3-base set) to the table and record the corresponding protein:

A U G = Methionine (start)

A A C = Asparagine

C A U = Histidine

U C A = Serine

G U A = Valine

U G G = Tryptophan

ORIGINAL DNA: A T G G G T C T A G C G A A A G A T

Complementary DNA:

mRNA DNA Molecule:

Amino Acid:

And we do it all again:

A T G G G T C T A G C G A A A G A T = original DNA

T A C C C A G A T C G C T T T C T A = complementry DNA

A U G G G U C U A G C G A A A G A U = mRNA

A U G = Methionine (start)

G G U = Glysine

C U A = Leucine

G C G = Alanine

A A A = Lysine

G A U = Aspartate

Explanation:

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