Biology, 13.10.2020 04:01 dreamer197
A group of peptides that influence nerve transmission in certain parts of the brain have been isolated from normal brain tissue. These peptides are known as opioids because they bind to specific receptors that also bind opiate drugs, such as morphine and naloxone. Opioids thus mimic some of the properties of opiates. Some researchers consider these peptides to be the brains own painkillers. Use the information below to determine the amino acid sequence of the opioid leucine enkephalin.
a. Complete hydrolysis by 6 M HCI at 110 degrees C followed by amino acid analysis indicated the presence of Gly, Leu, Phe, and Tyr in a 2:1:1:1 molar ratic.
b. Treatment of the peptide with 1-fluoro-2,4-dinitrobenzene followed by complete hydrolysis and chromatography indicated the presence of the 2,4-dinitrophenyl derivative of tyrosine. No free tyrosine could be found.
c. Complete digestion of the peptide with chymotrypsin followed by chromatography yielded free tyrosine and leucine, plus a tripeptide containing Phe and Gly in a 1:2 ratio.
Answers: 2
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Biology, 22.06.2019 07:30, claudioocampo5621
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A group of peptides that influence nerve transmission in certain parts of the brain have been isolat...
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