What do you think the title Blackfish means? What does it actually mean? Why is this an interesting choice as a film title? What are the required qualities to become a marine trainer?
How do the trainers continue to work at the park when they see the conditions the animals are kept in?
How do they compartmentalize their feelings?
Why are the trainers and the general public misinformed about whales? Should trainers be responsible in learning the career prior to marine park training?
Why is Tilikum still kept in marine parks when he’s proven to be an aggressive whale, causing liabilities to the park and a threat to trainers?
Is it a good idea to breed from an aggressive male? Will his genes be carried through to his offspring? What are the alternatives?
Why do experienced trainers seem to be the ones who have the most accidents and deaths in marine parks?
What should the solution be for the improvement of the health of the animals? What regulations should be enforced? What should the priority be for the trainer or for the animals or both? Explain.
Why hasn’t there been any changes made in trainer procedures in the last 20 years since the first trainer death occurred? What should be done?
Can marine parks create an environment where what happened at SeaWorld never happens again anywhere else?
Do we need marine park aquariums? Why do people visit them? Are they important or cruel? What are the alternatives?
Have you ever been to a marine park? Are you surprised at what you learned about them in the film? Would you visit a marine park aquarium after watching the film? Why or why not?

For your initial isolation of the bacterium, you simply needed to grow enough of it that you could identify some of its more general features. thus, you used a complex nutrient agar that would allow for the growth of almost any bacterial species. different bacterial species often have very specific requirements for life. this is a fact that you can utilize when you are trying to identify the bacterium to the species level. growth conditions that will permit the growth of one species but not another may allow you to select which one you would like to culture. you can utilize different selective/differential media that allow for the growth of only one type of bacteria (e. g., either gram positive or gram negative). culturing media such as phenylethyl alcohol or sodium chloride agar will be selective/differential for gram-positive bacteria. you may also want to show that it is different from other bacteria that may be present, so you could use a selective/differential agar. selective/differential media such as blood agar or phenol red sugar broths allow for the determination of whether a bacterial cell can grow and utilize specific ingredients included in the medium and turn the agar a distinct color. sheep's blood agar utilizes red blood cells to differentiate which bacterial species may have hemolytic properties. selective or differential? 1. an agar that uses a high salt concentration to limit the growth of one type of bacteria over another would be considered selective/differential. 2. if an agar contains a dye or ph indicator in the presence of a sugar, it is generally considered selective/differential for those bacteria that may ferment the sugar over those that cannot. 3. an agar that uses the dye in crystal violet agar suppresses the growth of gram-positive bacteria. this agar would be considered selective/differential. 4. the selective/differential aspect of macconkey agar allows for the determination of which bacteria are lactose fermenters and which are not. 5. a blood agar plate contains sheep red blood cells and allows for the determination of hemolytic capabilities for all bacteria that grow on the agar. this would make this type of agar selective/differential. 6. emb agar uses lactose and two dyes that allow it to be selective/differential between the blue-black colonies of e. coli and the pinkish colonies of all other enteric bacteria. 7. bile salts in macconkey agar allow for the agar to be selective/differential for the growth of enteric gram-negative bacteria over gram-positive bacteria. 8. an agar that allows for the distinction of bacteria based on metabolism would be considered selective/differential.

Which equation is used to calculate the magnetic force on a charge moving in a magnetic field

How are mutations continually being generated in a population (what are some of the causes of mutatuions? ) explain