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Biology, 21.09.2020 02:01 lolololol21

Targeting a DNA sequence for editing by the CRISPR‑Cas9 system requires that a protospacer‑adjacent motif (PAM) sequence appear just downstream of the target sequence. For S. pyogenes Cas9 this PAM is the trinucleotide NGG, where N represents any nucleotide. Assuming a fragment of DNA includes equal amounts of A, C, G, and T bases, how many bases apart, on average, would one expect to find the NGG PAM?

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Targeting a DNA sequence for editing by the CRISPR‑Cas9 system requires that a protospacer‑adjacent...

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