Donor Blood Type Recipient Blood Type A- Successful A+ A-
O- AB+
Would the above two transfusions be successful or would either of them harm the recipient? For each transfusion, explain your reasoning. In your explanations, use the following terms: antigen, antibodies, and agglutination.

The first blood transfusion in which the donor blood type is A+ and recipient blood type is A- would not be successful as the donor blood is A+, which signifies that the blood comprises both Rh factor and antigen A, while the recipient blood group is A-, which signifies that the blood does not comprise Rh factor and possess only antigen A. Therefore, at the time of transfusion, the Rh factor of donor blood will be regarded as a foreign body by the recipient, and thus, will generate antibody against the donor Rh factor provoking immune rejection and agglutination.  

On the other hand, the second blood transfusion in which the donor blood type is O- and the recipient blood type is AB+, the blood transfusion would be successful as the blood group O- is a universal donor, as it neither possesses any A or B antigen nor Rh factor. This blood group is accepted by all the blood group recipients.  

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.