Set up a mating between an Hfr strain and an F- (ade- his- bio-) strain and detect bio+ recombinant. Which of the following mating can let me select the recombinant by a single step (or a single selective medium)? 1. Hfr (ade + his+ bio+ Str-S) X F- (ade- his- bio-)
2. Hfr (ade+ his- bio-) X F- (ade- his- bio-)
3. Hfr (ade+ his+ bio+ Str-R) X F- (ade- his- bio- Str-S)
4. Hfr (ade+ his+ bio+ Str-S) X F- (ade- his- bio- Str-R)

8) animals respond in various ways to stimuli detected through their senses. some of these responses are stimulated by the presence of danger. which of these animal responses is the best example of a response to an immediate threat? a) a rooster crows every morning at the first sign of light. b) as something approaches an octopus, it shoots a cloud of ink. c) from late fall until spring, bears hibernate to preserve energy. d) a dog is getting hot in the sunlight, so he moves to a shaded area.