Imagine that you could microinject cytochrome c into the cytosol of both wild-type cells and cells that were lacking both Bax and Bak, which are apoptosis-promoting members of the Bcl2 family of proteins. Would you expect one, both, or neither of the cell lines to undergo apoptosis? Explain your reasoning.

Neither of the cell lines would undergo apoptosis

the cells will undergo apoptosis only if they have all the three BCl₃ family proteins. If bax and bak is absent means they can not form a channel to take out the cytochrome c. In such cells they can't follow the apoptosis.

Explanation:

Apoptosis will happen to the nutrient-deficient cells, cells that lost contact with the neighboring cells, etc. Cytochrome C can act as an apoptosis signal. Usually, cytochrome c will come out from the mitochondria. This is recognized by BCl₂ family proteins. This includes 3 subfamilies.

1) pro-apoptotic proteins (eg. bax, bak, bok)

2) anti-apoptotic proteins (eg. Bcl2, Bcl-XL, Bcl-W, etc)

3) BH₃ family proteins (eg. bad, bid, bim, etc).

The anti-apoptotic protein inhibit the apoptosis. When the cell reaches up to death, the BH₃ family proteins will inhibit the action of anti-apoptotic proteins. This will activate the pro-apoptotic proteins. Initially, the pro-apoptotic protein will stay in the mitochondrial membrane as monomers. When the cell needs death or apoptosis, these monomers will get organized and form a channel. These channels help to take apoptotic signals like cytochrome c to outside. And this leads to apoptosis.

easy

explanation: