With respect to mammalian cell culture, which of the following statements is true? During the establishment of a cell line, senescence is the last stage of the cell's life. Rodent cells cultures undergo spontaneous transformation with similar frequency to human cell cultures. Media used for cell growth contain proteases and a divalent cation chelator to help cells adhere to the surface. Embryonic cell lines do not undergo senescence nor do they have a finite life span.

Media used for cell growth contain proteases and a divalent cation chelator to help cells adhere to the surface.

Explanation:

Mammalian cell structure is the process where animals cells are grow in vitro in a flask or dish. The methods, equipments, reagents and supplies use in this type of culture prevent contamination and maintain cell culture purity. Protein enzymes like Serine protease breaks the cellular matrics interaction between the cells. In cell culture, the focal adhesions that are anchoring the cell to the culture dish is cut away. Most trypsin solutions for cell culture also contain EDTA which acts as a chelator for calcium. When calcium is remove from a solution, cadehrin that hold cells to each other break down and cells become separated from each other.