Energy that is not passed on from one trophic level to the next is

The amount of energy at each trophic level decreases as it moves through an ecosystem. As little as 10 percent of the energy at any trophic level is transferred to the next level; the rest is lost largely through metabolic processes as heat.

Explanation:

1. glaciers are melting

2. more water going into ocean (water's such as fresh waters, etc)

this protocol provides a detailed procedure for co-immunoprecipitation (co-ip) to identify protein–protein interactions.