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To determine whether regulation of gene expression by short RNAs was a naturally occurring phenomenon, researchers isolated RNA from a cell and fractionated them by size to obtain only short RNAs. The next step was to clone these molecules.
Today the cloning step would not be required. Which of the techniques below is the best reason why?
A. Because a microarray could tell if the fragments were encoded by the genome.
B. Because a PCR reaction could tell if the fragments were encoded by the genome.
C. Because an RNA-seq reaction could tell if the fragments were encoded by the genome.
D. Because a yeast 2 hybrid could tell if the fragments were encoded by the genome.
E. Because a GFP fusion could tell if the fragments were encoded by the genome.
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