Distinguish ways surface waters can be
polluted. Why is it important to understand
how each way impacts water quality?

You have been asked to lead a demonstration for the undergraduate microbiology lab course about the uses of negative staining when studying bacteria. a "negative" stain does not stain the bacterial cell itself but stains the space between cells. under magnification, the acidic (negativelycharged) nature of the stain will be repelled by the negatively charged bacterial cell wall and willleave the cell colorless in a stained background. negative stains are used primarily to reveal the presence of negatively charged bacterial capsules; therefore, they are also called capsule stains. encapsulated cells appear to have a halo surrounding them. the negative stain procedure does not require heat fixation, which limits any chances of alteration in bacterial cell shape and size. the bacterial suspension is added to a drop of stain, such as nigrosin or eosin, and drawn across the glass slide using a coverslip. nigrosin staining-not safranin staining-of klebsiella pneumoniae will allow for the visualization of the cell shape and the determination of the presence of a capsule. true/false

Match the following terms describing electrical events with the correct phases of the cardiac cycle. the ventricular muscle cells depolarize at the start of this phase. during this phase, na+ entry through the funny channels causes the pacemaker potential of sa nodal cells to gradually become less negative until it reaches threshold. the ventricular muscle cells repolarize right before this phase. the cytosolic concentration of calcium in the contractile cells of the ventricle is highest during this phase. a. isovolumetric relaxation b. ventricular ejection c. isovolumetric contraction d. ventricular filling

Which sentence is an example of a strong conclusion to an autobiography? a. i stayed after school three days in a row to practice onstage in the auditorium. b. i knew i would have to practice all week to memorize my dance for the recital. c. after the recital was over, i knew practicing was worth it, and i was looking forward to my next big challenge. d. on opening night, i danced in front of a packed auditorium.