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Biology, 11.05.2020 00:57 PeachyPeaches1489
Scientists studying transcription in yeast (Saccharomyces cerevisiae) created an experimental strain that produced a modified RNA polymerase containing a single amino acid substitution. The scientists determined the maximum elongation rate during transcription with and without the modified RNA polymerase enzyme (Figure 1 ).
The compound amanitin, which is commonly found in toxic mushrooms, is a specific RNA polymerase inhibitor. Amanitin binds to the RNA polymerase active site and inhibits transcription. In a second experiment, the scientists treated the wild-type and experimental strains of S. cerevisiae with a 40 μg/mL solution of amanitin and recorded the maximum elongation rate of the mRNA (Figure 2 ). Error bars represent ±2SEx¯ .
Identify the dependent variable in the experiments. Identify a control group missing from the second experiment. Justify the need for this control group in the second experiment.
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