Identify the plasmid that must contain the CD3y core promoter sequence but the fewest or no negative regulatory sequences. Based on the data in Figure 2, describe the most likely cause of the variation in luciferase activity among the cells that contain plasmids pCD3y-419, pCD3y-309, pCD3y-239, and pCD3y-199. Calculate the approximate percent increase in luciferase activity between cells containing plasmid pCD3y-59 and cells containing plasmid pCD3y-149. Round to the nearest whole number.