For some time it was not clear whether lac repressor inhibits lac operon transcription by inhibiting the binding of RNA polymerase to its promoter or by allowing transcription initiation but blocking elongation past the site of bound repressor.
How might you distinguish between these possibilities? Fill in the blanks with the options given to complete the following sentences.

in one gel, in separate gels, Northern blot, repressor, Western blot, inducer

Incubate a plasmid containing the lac operon with lac . Then add RNA polymerase and Northern blot analyze the mixture by gel electrophoresis and probe the gel with antibodies to RNA polymerase and repressor .

Incubate a plasmid containing the lac operon with lac __REPRESSOR. Then add RNA polymerase and Northern blot analyze the mixture by gel electrophoresis and probe the gel _WESTERN BLOT with antibodies to RNA polymerase and repressor _IN SEPERATE GELS.

Explanation:

Incubate a plasmid containing the lac operon with lac __REPRESSOR. Then add RNA polymerase and Northern blot analyze the mixture by gel electrophoresis and probe the gel _WESTERN BLOT with antibodies to RNA polymerase and repressor _IN SEPERATE GELS.

nb

explanation:

kj

passive transport is a movement of biochemicals and other atomic or molecular substances across cell membranes without need of energy input. unlike active transport, it does not require an input of cellular energy because it is instead driven by the tendency of the system to grow in entropy.

so, according to what that says, i believe it's hypertonic and hypotonic.

good luck!