Glyceraldehyde-3-phosphate dehydrogenase catalyzes the phosphorylation of glyceraldehyde-3-phosphate, but unlike other glycolytic reactions, it does not require energy investment from ATP. This reaction occurs in two stages: oxidation/reduction and phosphorylation.

Why is the oxidation/reduction reaction necessary to make phosphorylation favorable?

A. The oxidation/reduction reaction is necessary to oxidize phosphate, allowing addition to the substrate. B. The oxidation/reduction reaction is necessary to form an intermediate with a large free energy of hydrolysis. C. The oxidation/reduction reaction is necessary to produce NADH, which is required for ATP synthesis. D. The oxidation/reduction reaction is for the substrate to bind to the enzyme.

B. The oxidation/reduction reaction is necessary to form an intermediate with a large free energy of hydrolysis

Explanation:

An oxidation-reduction reaction is catalyzed by glyceraldehyde 3-phosphate dehydrogenase, oxidation of aldehyde to a carboxylic acid by NAD + and the joining of 3-phosphophoglycerate a carboxylic acid and orthophosphate to form the acyl-phosphate 1,3-biphosphoglycerate. This is done together so that aldehyde oxidation can be used to drive the formation of acyl phosphate. Energy that is released in the oxidation of glyceraldehyde 3-phosphate to 3-phosphoglycerate is trapped temporarily as 1,3-bisphosphoglycerate the energy aids the transfer of phosphoryl group from 1,3-phosphoglycerate to ADP to yield ATP.

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