Biology, 21.04.2020 17:39 Hehdjdvgs8464
An overproducer E. coli strain is an E. coli strain, which carries a recombinant plasmid with an ORF of a protein of interest under the control of a very strong promoter, therefore it produce large quantities of the protein of interest. For example, recombinant human insulin is produced using an overproducer E. coli strain, which carries a plasmid with the ORF of human insulin.
(a) You want to make an overproducer E. coli strain, which will make a protein from bacterium Salmonella, YFPS (your favorite protein from Salmonella). Below is a restriction map of a fragment of Salmonella genomic DNA, which contains ORF of YFPS and a restriction map of a plasmid, which you are using to make the overproducer. Which pair of restriction enzymes would you use?
b.) You want to use the same strategy to produce a human protein in E. coli cells, YFPH (your favorite protein from humans). You have two potential sources to make the insert: a fragment of human genomic DNA, which contains YFPH gene, and a fragment of human cDNA, which contains YFPH ORF. Which one would you use?
c.) What enzyme synthesizes DNA using mRNA as a template during cDNA construction?
Answers: 1
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Which of these is not an example of molecular homology? 1. use of dna and rna as genetic material 2.use of glycolysis as the first step in cellular respiration in both plants and animals 3. the lack of an igf-1 gene in prokaryotes 4. the use of aldolase b to break down fructose in bacteria, plants and animals
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Match the enzymes to their role in the dna replication process
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An overproducer E. coli strain is an E. coli strain, which carries a recombinant plasmid with an ORF...
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