Calculate your transformation efficiency. Transformation efficiency can be measured as total number transformants (also referred to as colony forming unit- cfu) per ug DNA used. For your transformation control, 100 pg of uncut pUC19 should be used (12.5 pg/uL provided to you). Our agar plate had about 400 colonies.

creative diagnostics provides a protocol for total protein extraction based on ripa which is widely used.

1. amino acid refers to the fundamental building block of proteins, comprising of an amino group and a carboxyl group.

2. catalyst refers to any substance, which increases the rate of chemical reaction without being used up in the reaction.

3. a complex molecule containing he genetic code is dna or deoxyribonucleic acid.

4. an enzyme refers to a molecule of protein, which is unique in accelerating up a particular chemical reaction in the biological systems.

5. nucleic acid refers to an sequence of sugars, phosphates, and nitrogenous bases, for example, dna and rna.

6. a protein refers to a large, composite organic molecule comprising of a huge number of amino acids associated together with the of peptide bonds.

7. rna refers to a nucleic acid that takes part in the production of proteins.

8. substrate refers to a compound, which takes part in a chemical reaction, and is chemically modified by the reaction.