You discover a strain of E. coli that is unable to grow using lactose as its carbon source. When you sequence its genome, you discover a 30 nucleotide deletion in the lac operon promoter. All other regulatory elements and genes are present and display the wild-type sequence. You want to introduce a plasmid that will restore normal lac operon function, i. e such that lactose induces expression of all 3 lactose metabolism enzymes. In addition to a promoter, what is the minimum set of genetic elements you would have to include on this plasmid to accomplish your goal?

Describe the change in phase and temperature as water moves from the oceans to the atmosphere and then returns to earth again as rain. a) evaporation : temperature increases; condensation : temperature decreases; precipitation (rain) : temperature decreases b) evaporation : temperature increases; condensation : temperature decreases; precipitation (rain) : temperature increases c) evaporation : temperature decreases; condensation : temperature decreases; precipitation (rain) : temperature decreases d) evaporation : temperature increases; condensation : temperature increases; precipitation (rain) : temperature decreases