The transport of a substance across a plasma membrane of a specific organelle requires energy. The rate at which the transport takes place also depends on temperature. A scientist isolated the specific organelle and then used the following treatments to determine the conditions that will result in the maximal transport. All treatments contained the extracted organelle and were maintained at 25°C. The data from this experiment indicate that maximal rate of transport of protein X at 25°C occurs at an ATP concentration of 1.0μm/mL. Which procedure should be done next to gather data needed to meet the scientist's objective?