Biology, 10.04.2020 16:29 kirsten8605
Mechanistically, DNA replication by a single replisome (with two DNA polymerases) at each replication fork represents a challenge, particularly on the lagging strand. Please select ALL correct statements describing the challenge and solution below.
A. Synthesis of the leading strand proceeds in the 3' to 5' direction, opposite from the 5' to 3' on the lagging strand.
B. There are fewer Okazaki fragments on the lagging strand as compared to the leading strand.
C. The challenge on the lagging strand is due to the directionality of DNA synthesis (5' to 3') required by DNA polymerase. On the lagging strand, synthesis would move the polymerase away from the replication fork.
D. Okazaki fragments are connected into a single strand of DNA by the formation of phosphodiester bonds catalyzed by DNA ligase.
E. The lagging strand requires a new RNA primer for each of the Okazaki fragment synthesized.
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