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Biology, 09.04.2020 20:01 sophiateaches053
DNA sequencing has transformed our understanding of genes, genomes and evolution. Which of the following statements comparing two common sequencing techniques, the chain termination method and next generation sequencing is TRUE?
A) The chain termination method is faster and more efficient, so it is used to generate large-scale sequences, while next generation synthesis is used for routine, small-scale jobs.
B) The chain termination method employs the polymerase chain reaction, but next generation sequencing does not.
C) In the chain termination method, the order of bases is detected by fluorescently labeling each dideoxy-nucleotide in a different color, while next generation sequencing determines the order of bases by detecting the release of PPi during the formation of the phosphodiester bond.
D) Next generation sequencing employs electrophoresis, but the chain termination method does not.
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