Predict the effect of a large amount of acetyl-CoA on the activity of pyruvate dehydrogenase and the rate of glycolysis. Note: pyruvate dehydrogenase is the enzyme complex that catalyzes the formation of acetyl-CoA from pyruvate. Predict the effect of a large amount of acetyl-CoA on the activity of pyruvate dehydrogenase and the rate of glycolysis. Note: pyruvate dehydrogenase is the enzyme complex that catalyzes the formation of acetyl-CoA from pyruvate. Pyruvate dehydrogenase activity will decrease while the rate of glycolysis will increase. Both pyruvate dehydrogenase activity and the rate of glycolysis will decrease. The rate of glycolysis will increase, thereby, leading to an increase in pyruvate dehydrogenase activity. Pyruvate dehydrogenase activity will increase while the rate of glycolysis will decrease.