What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I. Transform bacteria with a recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes (endonucleases). III. Extract plasmid DNA from bacterial cells. IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligase to seal plasmid DNA to non-plasmid DNA.

III. Extract plasmid DNA from bacterial cells

II. Cut the plasmid DNA using restriction enzymes (endonucleases)

IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.

V. Use ligase to seal plasmid DNA to non-plasmid DNA.

I. Transform bacteria with a recombinant DNA molecule.

Explanation:

In order to splice a foreign DNA into a plasmid and insert such plasmid into a bacterium, the following step-wise process has to be followed:

The correct order is III, II, IV, V, and I.

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answer: 45

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