Briefly discuss TWO potential pitfalls of culture independent techniques for studying microbial communities based solely on the sequences of 16S rRNA whose genes have been amplified by PCR. (Label your answers as (1) and (2). Only the first 2 answers will be considered; answers outside the allotted space immediately below the question will not be considered).

where in your body is there an enzyme that does function at a high or low ph? what is the name of that enzyme and its function?

1. amino acid refers to the fundamental building block of proteins, comprising of an amino group and a carboxyl group.

2. catalyst refers to any substance, which increases the rate of chemical reaction without being used up in the reaction.

3. a complex molecule containing he genetic code is dna or deoxyribonucleic acid.

4. an enzyme refers to a molecule of protein, which is unique in accelerating up a particular chemical reaction in the biological systems.

5. nucleic acid refers to an sequence of sugars, phosphates, and nitrogenous bases, for example, dna and rna.

6. a protein refers to a large, composite organic molecule comprising of a huge number of amino acids associated together with the of peptide bonds.

7. rna refers to a nucleic acid that takes part in the production of proteins.

8. substrate refers to a compound, which takes part in a chemical reaction, and is chemically modified by the reaction.