Pseudomonas sp. has a mass doubling time of 2.4 h when grown on acetate. The saturation constant using this substrate is 1.3 g/l (which is unusually high), and cell yield on acetate is 0.46 g cell/g acetate. If we operate a chemostat on a feed stream containing 38 g/l acetate, find the following:
a. Maximum dilution rate
b. Cell concentration when the dilution rate is one-half of the maximum
c. Substrate concentration when the dilution rate is 0.8 Dmax
d. Cell productivity at 0.8 Dmax
Answers: 1
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