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Biology, 04.04.2020 02:34 nancylagunas805

E. coil cells grown on 15 N medium are transferred to 14 N medium and allowed to grow for two generations. DNA extracted from these cells is ultracentrifuged in a cesium chloride density gradient. What density distribution of DNA would you expect in this experiment? (a) What did Meselson and Stahl observe when
(i) They cultured E. coli in a medium containing
15
NH
4

Cl for a few generations and centrifuged the content.
(ii) They transferred one such bacterium to the normal medium of NH
4

Cl and cultured for two generations.

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