Biology, 01.04.2020 17:54 tammydbrooks43

A cloning vector was digested with only one restriction enzyme. Immediately after a single ORF flanked with overhanging ends, generated with the same enzyme, was cloned into the plasmid. The ORF was inserted downstream a single inducible promoter. Which of the following is the correct cloning rate expected?Note: Only plasmids carrying inserts should be considered. a. 25%b. 10%c.100%d. 50%

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A cloning vector was digested with only one restriction enzyme. Immediately after a single ORF flank...