Substitution mutations in which region(s) of a eukaryotic gene are likely to be silent?

A non-coding region of a gene and introns encoding region of a gene are the substitution mutation in eukaryotis gene which are likely to be silent.

E option is the correct.

Explanation:

Silent mutation is a change in a nucleotide sequence in a gene which encodes a protein. Silent mutation do not cause any change in the amino acid sequence of the protein formed by the mutated gene.

They have been labelled as evolutionarily neutral.

The amino acid which even if gets replaced has same properties as the one which would have been encoded by the original triplet.

The substitution mutation is a change in nucleotide by getting their functional groups change as Adenine to Guanine.

The non-coding regions as intron if have substitution mutation it would be a silent mutation. Any single nucleotide mutation which disrupts slicing signal causes no protein formation.