Suppose that a scientist deletes the AAUAAA consensus sequence, the poly(A) tail, or the 5' cap from pre‑mRNAs in an mRNA. She then observes the effects on RNA regulation. Assign each RNA regulation consequence to the type of deletion most likely to result in the consequence.

1. AAUAAA deletion
2. Poly(A)‑tail deletion
3. 5' cap delatation

A. Introns are not removed from pre m-RNA
B. The pre-mRNA is not cleaved at the cleavage site
C. The mRNA is not transported to the cytoplasma

Explanation:

(A) introns are not removed from the pre-mRNA due to 5' deletion;

(B) the pre-mRNA is not cleaved at the cleavage site due to AAUAA deletion

(C) the mRNA is not transported to the cytoplasm due to poly A tail deletion;

AAUAA deletion:  if we delete the AAUAA  sequence then there is the change of sequence of pre- mRNA that the pre-mRNA is not cleaved at the cleavage site .

Poly(A) tail deletion: Poly(A) tail is the long tail of Adenine in the 3' end of mRNA, after the deletion of Poly(A) tail, it affects the transport of mRNA from the nucleus to the cytoplasm and  the mRNA is not transported to the cytoplasm if tail is deleted.

5' cap deletion; the effect of 5' cap deletion is that introns are not removed from the pre-mRNA. The process of intron removing and exon joining is called RNA splicing.

you are certainly talking about this picture:

a: is the label of the white square, it is showing the  replication fork, formed by helicase (d) ==> so it is the site of dna separation and replication 

b: is a new dna brand that is following directly the replication fork, so it is continuous ==> built continuously from 5' to 3

c: dna polymerase cannot replicate dna on its own, it needs the of their proteins, and one of them plays the role of a clamp which fixes  the dna with the dna template and increases  the processivity of the enzyme. ==>   stops polymerase from falling off the  template.

d: is the enzyme responsible for forming the replication fork (helicase) ==> unwinds and separates dna into two strands

e: is the delayed strand, it is built  in the opposite sense of the helicase, so dna will be built in fragments ==> built  fragments from 5' to 3 joins

and finally f: is the enzyme that builds  the new dna strand (dna polymerase) ==>   adds nucleotides to make a new dna helix.

credit: gradman555p98x9p

read more on -